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A through-focus series of the hilus region of the dentate gyrus. This movie illustrates some features of the direct 3-dimensional counting method. Step through the movie frame-by-frame. Each frame is separated by 1 µm in the z-axis, while the x- and y-axes are divided into 2 µm units. The red and green border is the counting box (really a cube), and the colored frames at the beginning and end of the movie are the faces of the cube parallel to the x-y plane.
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