Electronic Prenatal Mouse Brain Atlas, EPMBA
The Electronic Prenatal Mouse Brain Atlas, EPMBA, at present consists of two sets of annotated high-resolution images of coronal sections from Gestational Day (GD) 12 heads and GD 16 brains of C57BL/6J mice. Ten micron thick sections of paraffin embedded tissue were stained with Hematoxylin and Eosin. Images were prepared at various resolutions for annotations, high resolution presentation and to magnify (zoomify) an area of interest. 52 out of 280 sections of the GD 12 set were annotated and 72 out of 370 of the GD 16 set. Additionally, horizontal sections of a GD 12 head were aligned and re-assembled into a 3D volume for digital sectioning in arbitrarily oblique planes.
Mouse Brain Library Collection
The collection now consists of images from approximately 1500 brains. You can search the MBL for cases by strain, age, sex, body or brain weight.
Atlas of Developing Mouse Brain Gestational (Embryonic) Day 12: Schambra, Annotations Complete
Coronal sections of gestational day (GD) 12 mouse heads, every 5th. section, stained with a hematoxylin and eosin (H & E).
With the New Release of iScope Client Suite v1.2 now you can see over 154 Slides with an average of 550 Hi-resolution stacks for each slide.
As of Gumbo v2.7 and Aria3D v1.5, these two components of the iScope Client now feature more integration - specifically the ability to launch Aria3D from within Gumbo.
The MBL Process
This manual explains the process of embedding brain tissue in 12% celloidin, cutting the tissue with a microtome, staining and coverslipping the brain sections, the images of which are then posted on the internet for research purposes.
Horizonal A/J Atlas. This is a high resolution (2.6µm/pixel) atlas in 8-bit color
About this A/J Horizontal Atlas: The brain is that of a 365-day-old A/J female with a body weight of 22.8 grams and a brain weight (fixed) of 158.2 milligrams. In vivo brain weight was 386.4 mg. The animal's celloidin case ID number is 005 and you can view one of the two slides used to make this atlas. Shrinkage during fixation and processing is 25.6% (linear). The in vivo grid compensates for this shrinkage. The abbreviations we have used to label the sections conform to those in the Franklin-Paxinos atlas (The Mouse Brain in Stereotaxic Coordinates, Academic Press, San Diego, 1998, ISBN Number 0-12-266070-6).
Mouse Optic Nerve Image Database (MONID)
A collection of high magnification electron micrographs of the mouse optic nerve suitable for quantitative, comparative, and genetic analysis.